Home ContactUs 中文 English
main product:Sodium Dodecyl Sulfate
Yourlocation:Home >News> The interaction of sodium dodecyl sulfate with polyethylene oxide

The interaction of sodium dodecyl sulfate with polyethylene oxide

Time:2015/11/20 1:48:48

The interaction of sodium dodecyl sulfate with polyethylene oxide has been investigated by measurements of conductance, surface tension, and viscosity. The plots of surface tension and conductance as a function of the sodium dodecyl sulfate concentration show two transition points. These results are interpreted in terms of a polymer-surfactant complex or micelle formed by binding between the surfactant ions and the polymer.

Detection of calcium binding proteins by 45Ca autoradiography on nitrocellulose membrane after sodium dodecyl sulfate gel electrophoresis.

A new simple method of detecting calcium binding proteins in a protein mixture is described. A sample which might include calcium binding proteins was subjected to SDS-polyacrylamide gel electrophoresis and then electrophoretically transferred to a nitrocellulose membrane. The membrane was then incubated with 45Ca to detect calcium binding proteins as radioactive bands by autoradiography. Purified troponin-C, calmodulin, myosin DTNB light chain, and parvalbumin were clearly identified by this method. In the whole homogenate of chicken skeletal muscle, myosin DTNB light chain, troponin-C, and 55K calcium binding protein were found to be radioactive. In the frog skeletal muscle, small molecular weight proteins of approximately 13-15K and 70K protein appeared to be the calcium binding proteins. In the case of the carp skeletal muscle, small molecular weight proteins including parvalbumin and two proteins of about 80K seemed to bind calcium ion. Two high molecular weight calcium binding proteins were present in the scallop striated muscle. The procedure described can be completed within 24 h and can detect as little as 2 micrograms of calcium binding protein in the starting sample. Under appropriate conditions it was possible to detect only high affinity calcium binding proteins